standard concentration curve on excel for mac

Standard Concentration Curve On Excel Software Curve Expert
Standard Concentration Curve On Excel How To Calculate ELISA

If the list of coefficients exceeds the extent of the window that they are shown in, a scrollbar will appear to allow you to scroll through the parameters.

Read More News Company News Biological News Research Hotspots Promotions Events About Us About CUSABIO Quality Control Contact Us Home Technical Resources Research Tool ELISA Data Analysis.

ELISA data of samples can be interpolated from standard curve to calculate the concentrations of target proteins in samples.

However, we cant calculate the exact concentration since there isnt any standard protein in the kit.

When you analyze ELISA data with quantitative ELISA kit, you have to plot the mean absorbance against the protein concentration and draw a curve fitting your standard result best, and then interpolate absorbance of samples to the curve to calculate the concentration.

Standard Concentration Curve On Excel How To Calculate ELISA

This ELISA standard curve protocol will give you a comprehensive instruction on how to calculate ELISA results with quantitative ELISA kit step by step.

Standard Concentration Curve On Excel Software Curve Expert

We recommend you an ELISA data analysis software curve expert 1.3 or curve expert 1.4 that is simple to use and you can download at the end of this page to process data calculation.

There are many other curve fitting softwares available in the market to conduct ELISA calculation as well such as GraphPad Prism or you can also use normal MS Excel for analysis.

It is recommended to run standards and samples in duplicate or triplicate.

As a best practice, youd better control the CV of replicates less than 8.

Since sample diluent has absorbance even when there is no protein present at the detection wavelength, we run blank well with sample diluent in the assay to get the background OD.

It is common practice to subtract the absorbance of the blank well from standards and all sample absorbance.

Absorbance of both standards and samples are corrected by subtracting OD of blank well.

You can copy the data from excel sheet directly to the worksheet in the software.

If the polynomial family is included for consideration, you must specify the maximum degree of the polynomial that software will consider in the Polynomial Constraint area.

Of course, if the polynomial family is not included, the polynomial constraint will simply be ignored.

The following figures illustrate how different ELISA standard curves affect the accuracy of concentration calculations.

The closer r value is to 1, the stronger correlation there is between OD and concentration.

If a test sample produces an absorbance of 1.4, the corresponding two concentrations calculated by linear curve (r 0.92255796) and nonlinear curve (r 0.99993479) have a huge difference (199.955 VS 114.898).

By pressing the right mouse button or by pressing Ctrl-M in any graphing window, you can pick from the graphing menu.

In this example we would like to find the concentration of target protein (Y value) by evaluating the curve fit at a given OD (X value).

Just type in the point at which you want to evaluate the curve fit (the x value) in the At X field and press Calculate or Enter on your keyboard.

The most important information contained in this panel is the values of the parameters of the model.

These coefficients match the model shown in the Model section.

Standard Concentration Curve On Excel For Mac